hdac2 primary antibody  (Proteintech)

Journal: Biochemistry and Biophysics Reports

Article Title: The miR-146a-associated HDAC2 regulation of PI3K is involved in pancreatitis in vitro

doi: 10.1016/j.bbrep.2025.102057

Figure Lengend Snippet: The expression of HDAC2 and PI3K was detected by immunofluorescence double staining. In the immunofluorescent double staining for histone deacetylase 2 (HDAC2, red) and PI3K (green) in AR42J cells, (A):The merged image show the colocalization between them and immunoreactivities show that LPS-induced significant increase of HDAC2 and PI3K. (B): The semi-quantitative statistical analysis of immunodensity, ∗P < 0.05 compared with the control and #P < 0.05 compared with the LPS group.

Article Snippet: After dipping with PBS, the cells were blocked with 5 % BSA (A8020, Solarbio) at 37 °C for 30 min. Primary antibody HDAC2 (12922-3-AP, Proteintech, 1/200), and Cy3-labeled fluorescent secondary antibody (AS007, ABclonal, 1/200) was added in proper order.

Techniques: Expressing, Immunofluorescence, Double Staining, Histone Deacetylase Assay, Control

Journal: Biochemistry and Biophysics Reports

Article Title: The miR-146a-associated HDAC2 regulation of PI3K is involved in pancreatitis in vitro

doi: 10.1016/j.bbrep.2025.102057

Figure Lengend Snippet: Dual luciferase verified the targeting relationship between miR-146a and HDAC2. The renilla luciferase activity measured by luciferase detection reagent showed that the miR-146a can down-regulate HDAC2 gene in the 293T cells. (∗ indicates significant difference compared with HDAC2 WT group, P < 0.05, # indicates significant difference compared with HDAC2 WT + mimic NC).

Article Snippet: After dipping with PBS, the cells were blocked with 5 % BSA (A8020, Solarbio) at 37 °C for 30 min. Primary antibody HDAC2 (12922-3-AP, Proteintech, 1/200), and Cy3-labeled fluorescent secondary antibody (AS007, ABclonal, 1/200) was added in proper order.

Techniques: Luciferase, Activity Assay

Journal: Virology Journal

Article Title: Histone deacetylase III interactions with BK polyomavirus large tumor antigen may affect protein stability

doi: 10.1186/s12985-023-02128-6

Figure Lengend Snippet: HDAC3 and HDAC8 are required for BKPyV LT protein expression. A The expression of class I HDACs was knocked down by siRNA, then cells were infected with BKPyV and LT protein expression was determined by immunofluorescence and flow cytometric analysis 48 h post-infection. Middle panel: Quantification of the LT expression levels in the immunofluorescence assay. Right panel: LT protein expression was determined by intracellular staining and quantified by flow cytometry. B Class I HDACs were inhibited by HDAC inhibitors, cells were infected with BKPyV, and LT protein expression was determined by immunofluorescence and flow cytometric analysis 48 h postinfection. Middle panel: Quantification of the LT expression levels in the immunofluorescence assay. Right panel: LT protein expression was determined by intracellular staining and quantified by flow cytometry. BKPyV infection without siRNA or HDAC inhibitor treatment was considered 100%. (Green, LT protein; Red, Evan’s Blue stain). Data are representative of at least three independent experiments and are shown as the mean ± SD (* P < 0.05; ** P < 0.01; *** P < 0.001). (MFI: Mean fluorescence intensity)

Article Snippet: Primary antibodies against HDAC1, HDAC2, HDAC3, and HDAC8 were purchased from Cell Signaling Technology Inc. (Danvers, Massachusetts, USA).

Techniques: Expressing, Infection, Immunofluorescence, Staining, Flow Cytometry, Fluorescence